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Mapping of HIV-1 integrase preferences for target site selection with various oligonucleotides
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SYSNO ASEP 0333523 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mapping of HIV-1 integrase preferences for target site selection with various oligonucleotides Author(s) Snášel, Jan (UOCHB-X) RID
Rosenberg, Ivan (UOCHB-X) RID, ORCID
Pačes, Ondřej (UOCHB-X)
Pichová, Iva (UOCHB-X) RID, ORCIDNumber of authors 4 Source Title Archives of Biochemistry and Biophysics. - : Elsevier - ISSN 0003-9861
Roč. 488, č. 2 (2009), s. 153-162Number of pages 10 s. Language eng - English Country US - United States Keywords HIV integrase ; strand transfer reaction ; oligonucleotide substrates Subject RIV CE - Biochemistry R&D Projects 1M0508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06077 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000272541000009 DOI 10.1016/j.abb.2009.06.012 Annotation HIV integrase (IN) catalyzes the insertion of proviral DNA into the host cell chromosome. The integration site preference has been shown to be very diverse. We mapped the HIV integrase strand transfer reaction requirements using various short oligonucleotides (ON) that mimic the target DNA. We tested double stranded DNA dodecamers and hairpin-loop-forming oligonucleotides of different length. The integration biases with all target DNA structures tested were significantly influenced by changes in the resulting secondary ON structures. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2010
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