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Using Matlab in quantitative analysis of yeast growth
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SYSNO ASEP 0332092 Document Type K - Proceedings Paper (Czech conf.) R&D Document Type Conference Paper Title Using Matlab in quantitative analysis of yeast growth Title Použití Matlabu pro kvantitativní analýzu růstu kvasinek Author(s) Schier, Jan (UTIA-B) RID
Kovář, Bohumil (UTIA-B) RIDSource Title Technical Computing Prague 2009. - Praha : Humusoft, s.r.o, 2009 - ISBN 978-80-7080-733-0
S. 1-9Number of pages 9 s. Publication form www - www Action Technical Computing Prague 2009 Event date 19.11.2009 VEvent location Praha Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords image processing ; Petri dish ; Matlab ; image segmentation Subject RIV JC - Computer Hardware ; Software R&D Projects 1M0567 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z10750506 - UTIA-B (2005-2011) Annotation Quantitative growth analysis is used in microbiology, genetics and medical re- search to determine the in uence of the tested substance on the growth of colonies of some organism (we focus on yeast colonies in this paper). Examples of the tested substance include antibiotics, mutagens or medical drugs. To evaluate the experiment, time series of images of the colonies inoculated on Petri dishes are taken by digital camera in a darkroom. Then, relative area and the number of the colonies in each image have to be determined for further statistical processing. In the current laboratory practice, these parameters have often been hand-counted in each image, which is an error-prone process of a limited precision. Also, given the time demands of this hand-counting, the number of snapshots that can reasonably be processed is rather limited. In this paper, we introduce a Matlab-based tool for semi-automated processing of the dish images. Workplace Institute of Information Theory and Automation Contact Markéta Votavová, votavova@utia.cas.cz, Tel.: 266 052 201. Year of Publishing 2010
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