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The first detection of 'Candidatus Phytoplasma trifolii' in Rhododendron hybridum
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SYSNO ASEP 0330269 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The first detection of 'Candidatus Phytoplasma trifolii' in Rhododendron hybridum Title detekce 'Candidatus Phytoplasma trifolii' na Rododendron hybridum Author(s) Přibylová, Jaroslava (BC-A) RID, ORCID
Petrzik, Karel (BC-A) RID, ORCID
Špak, Josef (BC-A) RID, ORCIDNumber of authors 3 Source Title European journal of plant pathology. - : Springer - ISSN 0929-1873
Roč. 124, č. 1 (2009), s. 181-185Number of pages 5 s. Language eng - English Country NL - Netherlands Keywords Electron microscopy ; 16SrVI Clover proliferation group ; Phytoplasma Subject RIV EE - Microbiology, Virology R&D Projects 1QS500510558 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000264854700020 DOI 10.1007/s10658-008-9391-1 Annotation Four Rhododendron hybridum plants (from cvs Moravanka and Don Juan), all exhibited symptoms of shortened axillary shoots, reduced leaves with vein clearing and yellowing, undeveloped flowers, and general stunting in a rhododendron nursery garden in southern Bohemia in 2007. Electron microscopy examination of ultra-thin sections revealed numerous phytoplasma-like bodies in the phloem tissue of leaf midribs and petioles. The phytoplasma etiology of this disease was further confirmed by PCR using universal phytoplasma primers. RFLP analysis of PCR products obtained with a R16F2/R16R2 primer pair from all symptomatic plants indicated the presence of phytoplasma from the 16SrVI-A subgroup. A detailed comparison of the amplified sequences and phylogenetic analysis confirmed that the phytoplasma belonged to the subgroup 16SrVI-A (clover proliferation phytoplasma group). This is the first report of the natural occurrence of ‘Candidatus Phytoplasma trifolii’ in plants of Rhododendron hybridum. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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