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Quest for protein biomarkers of neural stem cells differentiation
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SYSNO ASEP 0329309 Document Type K - Proceedings Paper (Czech conf.) R&D Document Type Conference Paper Title Quest for protein biomarkers of neural stem cells differentiation Author(s) Skalníková, Helena (UZFG-Y) RID, ORCID
Halada, Petr (MBU-M) RID, ORCID
Vodička, Petr (UZFG-Y) ORCID
Motlík, Jan (UZFG-Y) RID, ORCID
Horing, O. (DK)
Jensen, O. N. (DK)
Gadher, S. J. (CH)
Pelech, S. (CA)
Kovářová, Hana (UZFG-Y) RID, ORCIDSource Title 1st Central and Eastern European Proteomic Conference and 3rd Czech Proteomic Conference. - Mělník : ÚŽFG, 2007
S. 9-9Number of pages 1 s. Action 1st Central and Eastern European proteomic Conference and 3rd Czech Proteomic Conference Event date 29.10.2007-31.10.2007 VEvent location Praha Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords neurogenesis ; cell diferentiation Subject RIV FH - Neurology R&D Projects LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 1M0538 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50450515 - UZFG-Y (2005-2011) AV0Z50200510 - MBU-M (2005-2011) Annotation Understanding neurogenesis and neural cell differentiation presents a unique challenge for treatment of nervous system disorders. To gain more insight about molecular mechanism of differentiation of neural cells, we applied different proteomic approaches using classical 2-DE followed by MS and antibody microarrays. Based on 2-DE, profile of constituent proteins of neural stem cells and their differentiated progenies was estabilished at first and then the protein species that are significantly up or down regulated during the differentiation were selected. Differentiation of neural cells was accompanied by changes in the expression of proteins involved in DNA and RNA binding, mRNA processing and transport, stress responses, iron storage and redox regulation. Immunoblot verified changes of hnRNP A1, hnRNP A2/B1, RafB, heme-oxygenasy 2, GRK2 proteins and alphaB-crystallin (S45), CDK1/2 (Y15), PKC mu (S738+S742), proline-rich Akt substrate (T246) phosphorylations during differentiation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
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