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The expresion of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line A549
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SYSNO ASEP 0328286 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title The expresion of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line A549 Title Exprese NADPH oxidáz a produkce reaktivních kyslíkových metabolitů buňkami plicního karcinomu A549 Author(s) Kolářová, Hana (BFU-R) RID
Pejchalová, Kateřina (BFU-R)
Kubala, Lukáš (BFU-R) RID, ORCIDSource Title Programme & Abstracts. - Brno, 2009 - ISSN 1337-6853
S. 112Number of pages 1 s. Action TOXCON 2009, 14th Interdisciplinary Toxicology Conference Event date 01.06.2009-03.06.2009 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords lung epithelial cells ; reactive oxygen species ; fluorometry Subject RIV AQ - Safety, Health Protection, Human - Machine R&D Projects GA524/06/1197 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50040507 - BFU-R (2005-2011) AV0Z50040702 - BFU-R (2007-2013) Annotation Controlled production of reactive oxygen species (ROS) by NADPH oxidases presented in non-phagocytic cells is recently suggested to significantly participate in regulation of cellular functions. We screened human lung epithelial cell line A549 for expression of NADPH oxidases NOX1, NOX4, DUOX1 and DUOX2 by real-time PCR. fluorimetric, colorimetric, and chemiluminometric methods were applied and optimized to determine ROS production. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2010
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