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Vascular endothelial cells on two- and three-dimensional fibrin assemblies for biomaterial coatings
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SYSNO ASEP 0325450 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Vascular endothelial cells on two- and three-dimensional fibrin assemblies for biomaterial coatings Title Vaskulární endoteliální buňky na dvou- a tří-dimenzionálních fibrinových strukturách pro povlékání biomateriálů Author(s) Filová, Elena (FGU-C) RID, ORCID
Brynda, Eduard (UMCH-V) RID
Riedel, Tomáš (UMCH-V) RID, ORCID
Bačáková, Lucie (FGU-C) RID, ORCID
Chlupáč, Jaroslav (FGU-C) ORCID, RID
Lisá, Věra (FGU-C)
Houska, Milan (UMCH-V) RID
Dyr, J. E. (CZ)Source Title Journal of Biomedical Materials Research. Part A. - : Wiley - ISSN 1549-3296
Roč. 90, č. 1 (2009), s. 55-69Number of pages 14 s. Language eng - English Country US - United States Keywords artificial fibrin networks ; surface modification ; nanostructure Subject RIV EI - Biotechnology ; Bionics R&D Projects IAA400500507 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 1QS500110564 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50110509 - FGU-C (2005-2011) AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000266648700006 DOI 10.1002/jbm.a.32065 Annotation Various techniques for coating synthetic surfaces with fibrin structures were tested for seeding bovine pulmonary artery endothelial cells (EC). Two-dimensional fibrin (Fb) structures (2D Fb) were obtained by successively repeating adsorption of fibrinogen (Fbg), incubating the surface with thrombin (Thr) solution, and inhibiting surface-attached Thr. Three-dimensional fibrin networks immobilized at the surface (3D Fb) were formed by catalytic action of surface-attached Thr on an ambient Fbg solution. 2D Fb layers effectively promoted initial spreading and proliferation of EC, but the maturation of EC on these surfaces was slow. Fast proliferation and maturation of EC were attained on thick 3D Fb. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2009
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