Short-term variation in extracellular phosphatase activity: Possible limitations for diagnosis of nutrient status in particular algal populations

Štrojsová, A.; Vrba, Jaroslav

doi:https://dx.doi.org/10.1007/s10452-007-9154-7

Number of the records: 1

Short-term variation in extracellular phosphatase activity: Possible limitations for diagnosis of nutrient status in particular algal populations

1.

SYSNO ASEP	0321203
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Short-term variation in extracellular phosphatase activity: Possible limitations for diagnosis of nutrient status in particular algal populations
Title	Krátkodobá variabilita extracelulární fosfatázové aktivity: Možná omezení pro diagnózu stavu živin v jednotlivých řasových populacích
Author(s)	Štrojsová, A. (CZ) Vrba, Jaroslav (BC-A)_{RID, ORCID}
Source Title	Aquatic Ecology. - : Springer - ISSN 1386-2588 Roč. 43, č. 1 (2009), s. 19-25
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	ELF97 phosphate ; ectoenzyme activity ; diurnal ; Ankyra ancora ; Chlorogonium fusiforme ; Monoraphidium dybowski
Subject RIV	EH - Ecology, Behaviour
R&D Projects	IAA6017202 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z60170517 - HBU-Z, BC-A (2005-2011)
UT WOS	000263159200002
DOI	10.1007/s10452-007-9154-7
Annotation	Species-specific extracellular phosphatase activity (PA) was studied in natural phytoplankton with an emphasis on short-term variation in PA and its consequences for the diagnosis of phosphorus (P) deficiency. PA was directly detected by epifluorescence microscopy using fluorescently labelled enzyme activity and quantified using image analysis. High short-term variation was found both in the total PA of the samples and specific PA of Ankyra ancora, Monoraphidium dybowski, and Chlorogonium fusiforme. The specific PA of these species varied between 0 and 157 fmol cell–1 h–1. The PA of Chlorogonium fusiforme showed a significant relationship with the time of day. Populations of the certain species were present that differed in physiological status and activity due to diurnal or random changes caused by mixing in lakes. Thus, species-specific extracellular PA should be interpreted carefully as an indicator of P deficiency in freshwater phytoplankton if based on limited number of samplings.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2009

Number of the records: 1