Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466

Koběrská, Markéta; Kopecký, Jan; Olšovská, Jana; Jelínková, Markéta; Ulanová, Dana; Man, Petr; Flieger, Miroslav; Janata, Jiří

doi:https://dx.doi.org/-

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Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466

1.

SYSNO ASEP	0315795
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466
Title	Analýza sekvence a heterologní exprese shluku pro biosyntézu linkomycinu z typového kmene Streptomyces lincolnensis ATCC 25466
Author(s)	Koběrská, Markéta (MBU-M)_ORCID Kopecký, Jan (MBU-M) Olšovská, Jana (MBU-M) Jelínková, Markéta (MBU-M) Ulanová, Dana (MBU-M) Man, Petr (MBU-M)_{RID, ORCID} Flieger, Miroslav (MBU-M)_ORCID Janata, Jiří (MBU-M)_{RID, ORCID}
Source Title	Folia Microbiologica. - : Springer - ISSN 0015-5632 Roč. 53, č. 5 (2008), s. 395-401
Number of pages	7 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	streptomyces lincolnensis ; cosmid ; lincomycin
Subject RIV	EE - Microbiology, Virology
R&D Projects	GA204/05/0616 GA ČR - Czech Science Foundation (CSF)
	1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000261763200002
DOI	-
Annotation	A cosmid bearing an insert of 38217 bp covering the gene cluster and its flanking regions type strain Streptomyces lincolnensis ATCC 25466 was sequenced. Two relatively extensive sequence changes and several hundred point mutations were identified if compared with the previously published sequence of the lincomycin industrial strain Streptomyces lincolnensis 78-11. Analysis of the cluster flanking regions revealed its localization within the genome of S. lincolnensis ATCC 25466. The cluster-bearing cosmid was integrated into the chromosome of lincomycin non-producing strains Streptomyces coelicolor CH 999 and Streptomyces coelicolor M 145. The modified strains heterologously produced lincomycin, but the level dropped to approximately 1-3% of the production in S. lincolnensis ATCC 25466
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2009

Number of the records: 1