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Trichostrongylus colubriformis rDNA polymorphism associated with arrested development
- 1.0315027 - FGÚ 2009 RIV DE eng J - Journal Article
Langrová, I. - Zouhar, M. - Vadlejch, J. - Borovský, M. - Jankovská, I. - Lytvynets, Andrej
Trichostrongylus colubriformis rDNA polymorphism associated with arrested development.
[Polymorfizmus rDNA Trichostrongylus colubriformis spojený s hypobiózou.]
Parasitology Research. Roč. 103, č. 2 (2008), s. 401-403. ISSN 0932-0113. E-ISSN 1432-1955
Institutional research plan: CEZ:AV0Z50110509
Keywords : arrested development * polymorphism * rDNA
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.473, year: 2008
PCR was performed on the cistron of rDNA in the two groups of infective larvae Trichostrongylus colubriformis —the population with and without ability to undergo arrested development. General primers designed by Caenorhabditis elegans rDNA were used for the amplification of the rDNA cistron between genes 18S and 28S. Amplified fragments were digested by using a series of restriction endonucleases. Hinc II restriction profiles unique for each T. colubriformis populations were observed, and therefore enzyme Hinc II appears to be useful for the determination of populations with and without the ability to undergo arrested development
PCR reakce byla provedena na cistronu rDNA u dvou skupin infekčních larev Trichostrongylus colubriformis - populace se schopností pozastavit vývoj a populace bez této schopnosti. Primery navržené pro rDNA Caenorhabditis elegans byly použity pro amplifikaci cistronu rDNA mezi geny 18S a 28S. Amplifikované úseky byly natráveny sérií restrikčních endonukleáz. U obou populací T. colubriformis byly po natrávení enzymem Hinc II sledovány unikátní profily. HincII by mohl být vhodným enzymem pro determinaci populací se schopností podstoupit hypobiózu a bez této schopnosti
Permanent Link: http://hdl.handle.net/11104/0165358
Number of the records: 1