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Hydrogen peroxide removal with magnetically responsive Saccharomyces cerevisiae cells
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SYSNO ASEP 0314835 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Hydrogen peroxide removal with magnetically responsive Saccharomyces cerevisiae cells Title Odstranění peroxidu vodíku pomocí buněk Saccharomyces cerevisiae vykazující magnetickou odezvu Author(s) Šafařík, Ivo (UEK-B) RID, SAI
Maděrová, Zdeňka (UEK-B) RID
Šafaříková, Miroslava (UEK-B) RID, SAISource Title Journal of Agricultural and Food Chemistry. - : American Chemical Society - ISSN 0021-8561
Roč. 56, - (2008), s. 7925-7928Number of pages 4 Language eng - English Country US - United States Keywords magnetic alginate beads ; catalase ; magnetic separation ; Saccharomyces cerevisiae cells ; hydrogen peroxide Subject RIV GM - Food Processing R&D Projects 2A-1TP1/094 GA MPO - Ministry of Industry and Trade (MPO) OC 157 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60870520 - UEK-B (2005-2011) Annotation Hydrogen peroxide (HP) is a promising chemical sanitizer for use in the food industry. Its residues have to be decomposed, usually using an enzyme process employing catalase. We have prepared magnetically responsive alginate beads containing entrapped Saccharomyces cerevisiae cells and magnetite microparticles. Larger beads (2-3 mm in diameter) were prepared by dropping the mixture into calcium chloride solution, while microbeads (50 - 100 μm in diameter) were prepared using the water in oil emulsification process. In general, microbeads enabled more efficient HP decomposition. The prepared microparticulate biocatalyst caused efficient decomposition of HP in water solutions (up to 2% concentration), leaving very low residual HP concentration after treatment (below 0.001% under appropriate conditions). The biocatalyst was stable; the same catalytic activity was observed after one month storage at 4 °C, and the microbeads could be used at least five times. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2009
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