Regulation of cap-dependent translation initiation in the early stage porcine parthenotes

0312613 - ÚŽFG 2009 RIV US eng J - Journal Article
Šušor, Andrej - Jelínková, Lucie - Karabínová, Pavla - Torner, H. - Tomek, W. - Kovářová, Hana - Kubelka, Michal
Regulation of cap-dependent translation initiation in the early stage porcine parthenotes.
[Regulace na čepičce závislé translační iniciace závislé v časném prasečím partenotu.]
Molecular Reproduction and Development. Roč. 75, č. 12 (2008), s. 1716-1725. ISSN 1040-452X. E-ISSN 1098-2795
R&D Projects: GA ČR GA524/04/0104; GA ČR GA524/07/1087
Institutional research plan: CEZ:AV0Z50450515
Keywords : meiosis * translation initiation * porcine parthenotes
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.287, year: 2008

In this study we document that both the translation initiation factor eIF4E and its regulator 4E-BP1 become dephosphorylated in the early stage porcine zygotes already 8 hr post-activation.Similarly, the activities of ERK1/2 MAP and Mnk1 kinases, which are both involved in eIF4E phosphorylation, gradually decrease during this period with the timing similar to that of eIF4E dephosphorylation. The formation of an active eIF4F complex is also diminished after 9-15 hr post-activation, although substantial amounts of this complex have been detected also 24 hr post-activation (2-cell stage). The overall protein synthesis in the parthenotes decreases gradually from 12 hr post-activation reaching a minimum after 48 hr (4-cell stage). Although the translation is gradually decreasing during early preimplantation development, the eIF4F complex, which is temporarily formed, might be a premise for the translation of a small subset of mRNAs at this period of development.

V této studii dokumentujeme, že translační iniciační faktor eIF4E a jeho regulátor 4E-BP1 jsou defosforylovány v časných prasečích zygotách již za 8 hod po aktivaci. Současně se snižuje aktivita ERK1/2 MAP and Mnk1 kináz. Tvorba aktivního eIF4F komplexu je rovněž snížena 9-15 hod po aktivaci ale za 24 hod je opět detekována jeho podstatná hladina. Proteosyntéza průběžně klesá do 48 hod.. Přestože je translace v preimplantačním stadiu průběžně snižována, může mít přechodná tvorba eIF4F komplexu vliv na translaci malého subsetu mRNA v této vývojové periodě.
Permanent Link: http://hdl.handle.net/11104/0163627