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Analysis of radiation-induced W Chromosome aberrations in the codling moth, Cydia pomonella (L.), by fluorescence in situ hybridization techniques
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SYSNO ASEP 0311986 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of radiation-induced W Chromosome aberrations in the codling moth, Cydia pomonella (L.), by fluorescence in situ hybridization techniques Title Analýza zářením indukovaných aberací chromosomu W u obaleče jablečného, Cydia pomonella (L.), technikami fluorescenční in situ hybridizace Author(s) Makee, H. (SY)
Tafesh, N. (SY)
Marec, František (BC-A) RID, ORCIDNumber of authors 3 Source Title Journal of Pest Science. - : Springer - ISSN 1612-4758
Roč. 81, č. 3 (2008), s. 143-151Number of pages 9 s. Language eng - English Country DE - Germany Keywords codling moth ; W chromosome ; W-chromosome painting probe Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA6007307 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA206/06/1860 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000258539600003 DOI https://doi.org/10.1007/s10340-008-0199-8 Annotation The structure of the female sex-determining W chromosome was examined in wild-type females and in three mutant female lines of the codling moth, Cydia pomonella. The mutant lines were isolated from progeny of irradiated females using the abnormal appearance of sex chromatin body in female somatic polyploid nuclei as a cytogenetic marker. A detailed cytogenetic analysis was carried out using three different fluorescence in situ hybridization techniques: genomic in situ hybridization (GISH), comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH) with the codling moth W-chromosome painting probe. The FISH techniques enabled us to identify the W chromosome and its fragments in mitotic and meiotic chromosome complements and thus helped us to resolve complicated W chromosome aberrations induced by irradiation. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2009
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