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Magnetic ovalbumin and egg white aggregates as affinity adsorbents for lectins separation
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SYSNO ASEP 0309615 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Magnetic ovalbumin and egg white aggregates as affinity adsorbents for lectins separation Title Magnetické agregáty ovalbuminu a vaječného bílku jako afinitní sorbenty pro separaci lektinů Author(s) Maděrová, Zdeňka (UEK-B) RID
Šafaříková, Miroslava (UEK-B) RID, SAI
Šafařík, Ivo (UEK-B) RID, SAISource Title Biochemical Engineering Journal. - : Elsevier - ISSN 1369-703X
Roč. 40, - (2008), s. 542-545Number of pages 4 s. Language eng - English Country NL - Netherlands Keywords egg white ; lectins ; magnetic protein aggregates Subject RIV EI - Biotechnology ; Bionics R&D Projects OC 157 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 2A-1TP1/094 GA MPO - Ministry of Industry and Trade (MPO) CEZ AV0Z60870520 - UEK-B (2005-2011) UT WOS 000257536600020 Annotation Simple and low-cost preparation of new magnetic adsorbents from ovalbumin and egg white, based on methanol precipitation and subsequent glutaraldehyde cross-linking has been developed. These adsorbents were used for preconcentration of two plant lectins from potato (Solanum tuberosum) tubers and from wheat (Triticum spp.) germs extracts. The adsorbed lectins were eluted with diluted hydrochloric acid. The specific activities of both lectins increased approximately 30–40 times during the preconcentration process. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2009
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