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LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147
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SYSNO ASEP 0307370 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147 Title LFA-1-zprostředkovaná adheze leukocytů je regulována interakcemi CD43 s LFA-1 a CD147 Author(s) Khunkaewla, P. (AT)
Schiller, H.B. (AT)
Paster, W. (AT)
Leksa, V. (SK)
Čermák, Lukáš (UMG-J) ORCID
Anděra, Ladislav (UMG-J) RID
Hořejší, Václav (UMG-J) RID
Stockinger, H. (AT)Source Title Molecular Immunology. - : Elsevier - ISSN 0161-5890
Roč. 45, č. 6 (2008), s. 1703-1711Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords leukocyte adhesion and aggregation ; monoclonal antibodies ; receptor signaling Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000254181800017 Annotation Activity of LFA-1 (CD11a/CD18) must be tightly controlled at onset of cellular immunity. Sialoglycoprotein CD43 can affect LFA-1-mediated cell adhesion in anti- or pro-adhesive manner. Using Y2H screen and coimmunoprecipitation we identified and characterized physical and functional association of CD43 with 2 novel partners, LFA-1 and CD147. Monoclonal antibodies to both CD43 and CD147 induced homotypic cell aggregation and adhesion of Jurkat T cells and U937 myeloid cells as well as dynamic co-capping of LFA-1 together with CD43 and CD147 to cell contact zones. Cotransfection of CD43 interfered with CD147-induced cell adhesion and aggregation, and siRNA-mediated knock down of CD43 in human T cells led to enhanced LFA-1 activation induced via CD147 and T cell antigen receptor. Triggering CD43 and underlying signaling pathways thus seems to enhance LFA-1 adhesiveness. CD43 also negatively regulates LFA-1 induction via other receptors by dynamic interaction with either LFA-1 or CD147. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2009
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