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A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo
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SYSNO ASEP 0306812 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo Title Jednoaminokyselinová substituce v receptoru TvbS1 snižuje susceptibilitu k infekci ptačími sarkomovými a leukosovými viry podskupin B a D a podmiňuje resistenci k podskupině E in vitro a in vivo Author(s) Reinišová, Markéta (UMG-J)
Šenigl, Filip (UMG-J) RID
Yin, X. (CZ)
Plachý, Jiří (UMG-J) RID
Geryk, Josef (UMG-J) RID
Elleder, Daniel (UMG-J) RID
Svoboda, Jan (UMG-J) RID
Federspiel, M. J. (US)
Hejnar, Jiří (UMG-J) RIDSource Title Journal of Virology - ISSN 0022-538X
Roč. 82, č. 5 (2008), s. 2097-2105Number of pages 9 s. Language eng - English Country US - United States Keywords retrovirus receptors ; avian sarcoma and leukosis virus ; resistance to retrovirus Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA523/07/1171 GA ČR - Czech Science Foundation (CSF) GA523/07/1282 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000253481000006 Annotation ASLV family retroviruses contain 5 highly related envelope subgroups (A-E). Susceptibility or resistance of cells to infection by these ASLVs is given by 3 loci in chicken. The tvb gene encodes subgroup B, D, E ASLV receptors. Wild-type TvbS1 receptor confers susceptibility to subgroup B, D, E ASLVs. In this study the genetic defect underlying altered susceptibility of inbred chicken line M to infection by ASLV(B), (D), (E), C125S substitution in tvb-encoded TvbS1 receptor in this line, was identified. We show that this substitution significantly reduces susceptibility of line M cells to infection by ASLV(B) and (D) and virtually eliminates susceptibility to ASLV(E) infection in cultured cells and the incidence and growth of ASV-induced sarcomas in chickens. It also significantly reduces binding affinity of TvbS1 receptor for subgroup B, D, E ASLV envelope glycoproteins. These results are first demonstration of possible role of the Cys-rich domain 3 in the function of Tvb receptors. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2009
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