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Detection of apoptotic DNA ladder in pig leukocytes and its precision using LM - PCR (ligation mediated polymerase chain reaction)
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SYSNO ASEP 0213487 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Detection of apoptotic DNA ladder in pig leukocytes and its precision using LM - PCR (ligation mediated polymerase chain reaction) Author(s) Matalová, Eva (UZFG-Y) RID
Španová, A. (CZ)Source Title Acta veterinaria Brno. - : Veterinární a farmaceutická univerzita Brno - ISSN 0001-7213
Roč. 71, č. 3 (2002), s. 163-168Number of pages 6 s. Language eng - English Country CZ - Czech Republic Keywords apoptosis ; phagocytosis ; DNA ladder Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA304/01/0850 GA ČR - Czech Science Foundation (CSF) KSK6005114 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Annotation Apoptosis as a way of programmed cell death represents an extremely important phenomen in development and everyday maintenance of homeostatis in multicellular organism. Regarding the keen interest in programmed cell death in the last ten years, more and more assays and kits are emerging to prove apoptosis. LM - PCR techniques has been shown not only as a method for result precision in the case of negative findings by DNA ladder assay but also as an effective assay in case of non-typical apoptotic DNA ladder appearance. Moreover, LM - PCR detected an apoptotic ladder even in populations with less than 1% of apoptotic cells. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2003
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