Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
1.
SYSNO ASEP
0191701
Document Type
C - Proceedings Paper (int. conf.)
R&D Document Type
Conference Paper
Title
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
Author(s)
Fučík, Vladimír (UMG-J) Šindelka, Radek (UMG-J) Beran, Jaroslav (UMG-J) Ferjentsik, Zoltán (UMG-J) Jonák, Jiří (UMG-J)
Source Title
Functional Genomics a Disease, Final programme and Abstract book. - Praha : ESF IN FUNCTIONAL GENOMICS, 2003
Pages
s. 93
Number of pages
1 s.
Action
ESF IN FUNCTIONAL GENOMICS: CONFERENCE /1./
Event date
14.05.2003-17.05.2003
VEvent location
Praha
Country
CZ - Czech Republic
Event type
WRD
Language
eng - English
Country
CZ - Czech Republic
Keywords
Mus musculus, c-src introns, microsatellite
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
GA301/02/0408 GA ČR - Czech Science Foundation (CSF)
CEZ
AV0Z5052915 - UMG-J
Annotation
In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in specific intervals. As the 31 bp interlink can assume a hairpin structure, the whole region can be viewed as an imperfect inverted repeat. The microsatellite was sequenced in imbred lines of M. m. musculus and M. m. domesticus, as well as in mice of both subspecies trapped near the hybrid zone. Differences in the length of repeat regions, insertion/deletions and simple base changes characterize the strains. Hybrids between the two species could be identified by sequencing of cloned PCR products. The structure of the microsatellite in more distant species was found to be conspicuously different. Instead of frequent (CA)18-25, we can see only (CA)8 in M. spretus, CATGCGCCCCCCA in Mus pahari and CACCCCCC in Mus caroli. No similar structure is present in the corresponding human src intron.
