Exogenous administration of gangliosides inhibits FcepsilonRI-mediated mast cell degranulation by decreasing the activity of phospholipase Cgamma

0191605 - UMG-J 20033110 RIV US eng J - Journal Article
Dráberová, Lubica - Dudková, L. - Boubelík, Michael - Tolarová, Helena - Šmíd, F. - Dráber, Petr
Exogenous administration of gangliosides inhibits FcepsilonRI-mediated mast cell degranulation by decreasing the activity of phospholipase Cgamma.
Journal of Immunology. Roč. 171, č. 7 (2003), s. 3585-3593. ISSN 0022-1767. E-ISSN 1550-6606
R&D Projects: GA MŠMT LN00A026; GA ČR GA204/00/0204; GA ČR GA204/03/0594; GA ČR GA310/00/0205; GA ČR GA301/03/0596; GA AV ČR IAA5052005; GA AV ČR IAA7052006; GA AV ČR IAA5052310; GA MZd NB6758
Institutional research plan: CEZ:AV0Z5052915
Keywords : mast cell * plasma membrane * IgE
Subject RIV: EC - Immunology
Impact factor: 6.702, year: 2003

In this study we analyzed the molecular mechanisms of an inhibition of mast cell activity by gangliosides. Rat basophilic leukemia cells pretreated with isolated brain gangliosides exhibited decreased secretory response and calcium release. Although the production of phosphoinositides was also decreased, activity of phosphatidylinositol 3-kinase was not affected. Gangliosides had no effect on association of aggregated FcepsilonRI with lipid rafts and on tyrosine phosphorylation of the receptor, Syk kinase and LAT adaptor. However, they inhibited enzymatic activity of phospholipase Cgamma. This resulted in decreased production of inositol 1,4,5-trisphosphate and an inhibition of calcium response. The combined data suggest that gangliosides inhibit formation of plasma membrane-associated signaling assemblies containing phospholipase Cgamma but not early activation events.
Permanent Link: http://hdl.handle.net/11104/0087345