Cultured human keratinocytes and tissue engineered skin substitutes

0191554 - UMG-J 20033054 RIV DB eng M - Monography Chapter
Matoušková, Eva - Brož, L. - Veselý, Pavel - Königová, R.
Use of allogeneic human keratinocytes cultured on dried porcine dermis in the treatment of burns.
Cultured human keratinocytes and tissue engineered skin substitutes. Stuttgart: Georg Thieme Verlag, Stuttgart, Germany, 2001, s. 230-238
Institutional research plan: CEZ:AV0Z5052915
Keywords : Allogeneic * Cultured * Burns
Subject RIV: EB - Genetics ; Molecular Biology

The absence of the dermal component predisposes sultured keratinocyte sheets to instability, contractibility, and difficult handling. In order to overxome these disadvantages, we developed recombined human/pig skin (RHPS) composed of human keratinocytes cultured on acellular pig dermis. The RHPS is applied "upside-down", with the epidermal cells facing the wound. Thus RHPS serves in this way as an effective keratinocyte delivery system. It has been proved that keratinocytes can migrate from the inverted RHPS with allogeneic keratinocytes allows early grafting without riquiring dispase digestion to release the graft from the dish.
Permanent Link: http://hdl.handle.net/11104/0087297