Cell cycle dependent expression of Plk 1 in synchronized porcine fetal fibroblasts
1.
SYSNO ASEP
0173866
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Cell cycle dependent expression of Plk 1 in synchronized porcine fetal fibroblasts
Author(s)
Anger, M. (US) Kues, W. A. (DE) Klíma, J. (CZ) Mielenz, M. (DE) Kubelka, M. (CZ) Motlík, J. (CZ) Ešner, M. (CZ) Dvořák, Petr (UEM-P) Carnwath, J. W. (DE) Niemann, H. (DE)
Source Title
Molecular Reproduction and Development
- ISSN 1040-452X
Roč. 65, - (2003), s. 245-253
Number of pages
9 s.
Language
eng - English
Country
US - United States
Keywords
serum deprivation
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
LN00A065 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ
AV0Z5039906 - UEM-P
Annotation
Enzymes of the Polo-like kinase family are active in the pathways controlling mitosis in several species. CDNA fragments of the porcine homologues of Plk1, Plk2, and Plk3 were cloned. All three cDNAs show high sequence homology with their mouse and human counterparts and contain the Polo box. In synchronized porcine fetal fibroblasts, Plk1 mRNA was barely detectable in G0 and G1, increased during S phase and peaked after G2/M transition. Porcine Plk1 protein was detected at the beginning of S phase and was highest after the G2/M transition. In summary, the Plk1 expression pattern in the pig is similar to that reported for other species.
