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Biochemical evidence for the activation of distinct subsets of mitogen-activated protein kinases by voltage and defense-related stimuli
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SYSNO ASEP 0172264 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Biochemical evidence for the activation of distinct subsets of mitogen-activated protein kinases by voltage and defense-related stimuli Author(s) Link, V. (DE)
Hofmann, M. (DE)
Sinha, A. (DE)
Ehness, R. (DE)
Strnad, Miroslav (UEB-Q) RID, ORCID
Roitsch, T. (DE)Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 128, č. 1 (2002), s. 271-281Number of pages 11 s. Language eng - English Country US - United States Keywords TOBACCO SUSPENSION CULTURE ; CYCLIN DEPENDENT KINASES ; CYTOSOLIC CALCIUM ION Subject RIV CE - Biochemistry R&D Projects OC 844.10 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z5038910 - UEB-Q Annotation Activation of mitogen-activated protein (MAP) kinases is a common reaction of plant cells in defense-related signal transduction pathways. To gain insight into the mechanisms that determine specificity in response to a particular stimulus, a biochemical approach has been employed. Photoautotrophic suspension culture cells of tomato (Lycopersicon peruvianum) were used as experimental system to characterize MAP kinase activation by different stress-related stimuli. An elicitor preparation of the tomato-specific pathogen Fusarium oxysporum lycopersici was shown to result in the simultaneous induction of four kinase activities that could be separated by ion-exchange chromatography. The simultaneous activation of multiple MAP kinases was further substantiated by distinct pharmacological and immunological properties: a differential sensitivity toward various protein kinase inhibitors and a differential cross-reaction with isoform-specific MAP kinase antibodies. In contrast to the two fungal elicitors chitosan and the F. oxysporum lycopersici preparation, the plant-derived stimuli polygalacturonic acid and salicylic acid were shown to activate distinctly different subsets of MAP kinases. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2003
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