Number of the records: 1  

Induced DNA damage measured by the Comet assay in 10 weed species

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    SYSNO ASEP0172228
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleInduced DNA damage measured by the Comet assay in 10 weed species
    Author(s) Gichner, Tomáš (UEB-Q)
    Mühlfeldová, Zdeňka (UEB-Q)
    Source TitleBiologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
    Roč. 45, č. 4 (2002), s. 509-516
    Number of pages8 s.
    Languageeng - English
    CountryCZ - Czech Republic
    KeywordsArabidopsis thaliana ; Agropyron repens ; Bellis perennis
    Subject RIVEB - Genetics ; Molecular Biology
    R&D ProjectsGA521/02/0400 GA ČR - Czech Science Foundation (CSF)
    CEZAV0Z5038910 - UEB-Q
    AnnotationFor most plant species growing in polluted areas no mutagenicity assays are available. We have studied the possibility of using the alkaline protocol of the Comet assay as a method for detecting induced DNA damage in wildly growing weeds. The monofuctional alkylating agent ethyl methanesulphonate (EMS) was applied on leaves of 10 weed species (ordered according to the diameter of the nuclei): Arabidopsis thaliana, Convolvulus arvensis, Bellis perennis, Urtica dioica, Lamium album, Chenopodium rubrum, Plantago media, Poa annua, Taraxacum officinale, and Agropyron repens. With increasing concentrations of EMS (2 to 10 mM) the DNA damage, expressed by the averaged median tail moment values, significantly increased in nuclei of all weeds studied. Using the Head Extent parameter of the Komet version 3.1, we have measured the diameter size of the nuclei of the 10 weed species either immediately after the isolation of the nuclei or after 20 or 45 min of treatment with alkaline buffer (pH > 13).
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Year of Publishing2003

Number of the records: 1  

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