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Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection
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SYSNO ASEP 0162439 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection Author(s) Šlais, Karel (UIACH-O) RID, ORCID
Horká, Marie (UIACH-O) RID, ORCID
Nováčková, J. (CZ)
Friedl, Z. (CZ)Source Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 23, č. 11 (2002), s. 1682-1688Number of pages 7 s. Language eng - English Country DE - Germany Keywords capillary isoelectric focusing ; fluorescence detection ; pI standards Subject RIV CB - Analytical Chemistry, Separation R&D Projects IAA4031901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z4031919 - UIACH-O Annotation We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2004
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