3D structure of the human genome: order in randomness

0126873 - BFU-R 20023173 RIV DE eng J - Journal Article
Kozubek, Stanislav - Lukášová, Emilie - Jirsová, Pavla - Koutná, I. - Kozubek, Michal - Cafourková, Alena - Bártová, Eva - Falk, Martin - Paseková, R.
3D structure of the human genome: order in randomness.
Chromosoma. Roč. 111, č. 5 (2002), s. 321-331. ISSN 0009-5915. E-ISSN 1432-0886
R&D Projects: GA AV ČR KSK5052113; GA AV ČR IBS5004010; GA MZd NC5955; GA ČR GA202/01/0197; GA ČR GA301/01/0186
Institutional research plan: CEZ:AV0Z5004920
Keywords : nuclear architecture * human genom * chromosome structure
Subject RIV: BO - Biophysics
Impact factor: 2.829, year: 2002

A complex study of the spatial arrangement of different genetic elements (genes, centromeres and chromosomal domains) in the cell nucleus is presented and the principles of this arrangement are discussed. We show that the radial location of genetic elements in the three-dimensional (3D) space between the center of the nucleus and the nuclear membrane is element specific and dependent on the position of the element on the chromosome. In contrast, mutual angular positioning of both homologous and heterologous genetic elements is, in the majority of cases, random. In several cases, tethering of heterologous genetic elements was observed. This close proximity of specific loci may be responsible for their mutual rearrangement and the development of cancer.
Permanent Link: http://hdl.handle.net/11104/0025100