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Application of ARDRA and PLFA analysis in characterizing the bacterial communities of the food, gut and excrement of saprophagous larvae of Penthetria holosericea (Diptera: Bibionidae): a pilot study
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SYSNO ASEP 0105405 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Application of ARDRA and PLFA analysis in characterizing the bacterial communities of the food, gut and excrement of saprophagous larvae of Penthetria holosericea (Diptera: Bibionidae): a pilot study Title Použití analýzy ARDRA a PLFA pro charakterizaci bakteriálního společenstva potravy, střeva a exkrementů saprofágní larvy Penthetria holosericea (Diptera: Bibionidae): pilotní studie Author(s) Oravecz, O. (HU)
Elhottová, Dana (UPB-H)
Krištůfek, Václav (UPB-H)
Šustr, Vladimír (UPB-H)
Frouz, Jan (UPB-H)
Tříska, Jan (UEK-B) RID, ORCID, SAI
Márialigeti, K. (HU)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 49, č. 1 (2004), s. 83-93Number of pages 11 s. Language eng - English Country CZ - Czech Republic Keywords ARDRA ; PLFA analysis ; bacterial community Subject RIV EH - Ecology, Behaviour R&D Projects IAB6066903 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA526/99/P033 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z6066911 - UPB-H, BC-A Annotation Amplified ribosomal DNA restriction analysis (ARDRA) was used to compare the bacterial communities of the food, the gut sections (ceca, anterior and posterior midgut, hindgut) and the excrement of the litter feeding bibionid larvae of Penthetria holosericea. For universal eubacterial primers ARDRA patterms were complex with only minor differences among samples. Taxon specific primers were also applied to characterize the samples. Fragment composition was transformed to presence/absence binary data and further analyzed. Cluster analysis revealed that bacterial communities of gut highly resembled each other with the exception of the ceca. ARDRA patterns of consumed leaves clustered together with the intact leaves but differed from those of the excrement. ARDRA results were compared with microbial community structure based on phospholipid fatty acid (PLFA) fingerprints. The cluster analysis of PLFA (presence/absence binary) data resulted in a pattern similar to the ARDRA data. The PCA analysis of PLFA relative content separated microbial communities into five groups: (1) anterior and posterior midgut, (2) hindgut, (3) ceca, (4) consumed and intact litter, (5) excrement. Both methods indicated that conditions in the larval gut result in formation of a specific microbial community which differs from both the food and excrement ones. Particularly ceca - (blind appendages, harbor very specific microbial community) are divided from the rest of the gut by perithropic membrane. Workplace Institute of Soil Biology (until 2005) Contact Ivana Chytková, chytkova@upb.cas.cz, Tel.: 387 775 753 Year of Publishing 2005
Number of the records: 1