Number of the records: 1  

Representing GC variation along eukaryotic chromosomes

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    SYSNO ASEP0105339
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleRepresenting GC variation along eukaryotic chromosomes
    TitleZobrazení variace GC v eukaryotických chromozomech
    Author(s) Pačes, Jan (UMG-J) RID, ORCID
    Zíka, Radek (UMG-J)
    Pačes, Václav (UMG-J) RID
    Pavlíček, A. (US)
    Clay, O. (IT)
    Bernardi, G. (IT)
    Source TitleGene. - : Elsevier - ISSN 0378-1119
    Roč. 333, - (2004), s. 135-141
    Number of pages7 s.
    Languageeng - English
    CountryNL - Netherlands
    KeywordsGC content ; genome organization ; compositional homogenity
    Subject RIVEB - Genetics ; Molecular Biology
    R&D ProjectsLN00A079 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z5052915 - UMG-J
    AnnotationGenome sequencing now permits direct visual representation, at any scale, of GC heterogeneity along the chromosomes of several higher eukaryotes. Plots can be easily obtained from the chromosomal sequences, yet sequence releases of mammalian or plant chromosomes still tend to use small scales or window sizes that obscure important large-scale compositional features. To faithfully reveal, at one glance, the compositional variation at a given scale, we have devised a simple scheme that combines line plots with color-coded shading of the regions underneath the plots. The scheme can be applied to different eukaryotic genomes to facilitate their comparison, as illustrated here for a sample of chromosomes chosen from seven selected species. As a complement to a previously published compact view of isochores in the human genome sequence [FEBS Lett. 511 (2002a) 165], we include here an analogous map for the recently sequenced mouse genome, and discuss the contribution of repetitive DNA to the GC variation along the plots. Supplementary information, including a database of color-coded GC profiles for all recently sequenced eukaryotes and the program draw_chromosomes_gc.pl used to obtain them, are available at http://genomat.img.cas.cz
    WorkplaceInstitute of Molecular Genetics
    ContactNikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
    Year of Publishing2010

Number of the records: 1  

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