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Sensitive electrochemical determination of unlabeled MutS protein and detection of point mutations in DNA
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SYSNO ASEP 0104693 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Sensitive electrochemical determination of unlabeled MutS protein and detection of point mutations in DNA Title Elektrochemické stanovení neznačeného proteinu MutS a detekce bodových mutací v DNA Author(s) Paleček, Emil (BFU-R) RID, ORCID
Masařík, Michal (BFU-R)
Kizek, René (BFU-R)
Kuhlmeier, D. (DE)
Hassmann, J. (DE)
Schülein, J. (DE)Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 76, č. 19 (2004), s. 5930-5936Number of pages 7 s. Language eng - English Country US - United States Keywords MutS protein ; DNA repair ; mercury electrodes Subject RIV BO - Biophysics R&D Projects IBS5004355 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KSK4055109 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5004920 - BFU-R Annotation MutS protein plays an important role in the DNA repair system in prokaryotic and eukaryotic cells; it recognizes unpaired and mispaired bases in duplex DNA and can be used for detection of point mutations in vitro. We have shown that small amounts of this protein can be detected electrochemically at mercury and carbon electrodes without any labeling. Using constant current stripping analysis (CPSA) and mercury electrodes, tens of attomoles of this protein can be detected. The sensitivity of the determination at carbon electrodes is by more than 3 orders of magnitude lower. Using biotinylated DNA duplexes attached to magnetic beads, single-base mismatches and insertion/deletions were recognized by MutS. Picogram amounts of this protein were detected by CPSA after MutS releasing from the beads. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2005
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