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High-affinity binding of tumor supressor protein p53 and HMGB1 protein to hemicatenated DNA loops

  1. 1.
    SYSNO ASEP0104679
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JOstatní články
    NázevHigh-affinity binding of tumor supressor protein p53 and HMGB1 protein to hemicatenated DNA loops
    Překlad názvuVysoce afinitní vazba nádorově supresorového proteinu p53 a HMGB1 s hemikatenátovými smyčkami DNA
    Tvůrce(i) Štros, Michal (BFU-R) RID, ORCID
    Muselíková Polanská, Eva (BFU-R)
    Pospíšilová, Š. (CZ)
    Strauss, F. (FR)
    Zdroj.dok.Biochemistry. - : American Chemical Society - ISSN 0006-2960
    Roč. 43, č. 22 (2004), s. 7215-7225
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovap53 ; HMGB1 ; DNA hemicatenane
    Vědní obor RIVBO - Biofyzika
    CEPIAA5004105 GA AV ČR - Akademie věd
    GA301/02/0952 GA ČR - Grantová agentura ČR
    KSK4055109 GA AV ČR - Akademie věd
    CEZAV0Z5004920 - BFU-R
    AnotaceWe have observed that chromatin architectural protein HMGB1 and tumor suppressor protein p53 could bind with high affinity to a novel DNA structure that forms a DNA loop maintained at its base by a hemicatenane (termed hcDNA). The affinity of p53 for hcDNA is 40-fold higher (Kd ~ 0.5 nM) than for its natural specific binding sites within its target genes (Mdm2 promoter). Binding of p53 to hcDNA remains detectable in the presence of up to ~4 orders of magnitude of mass excess of competitor linear DNA, suggesting a high specificity of the interaction. p53 displays a higher affinity for hcDNA than for DNA minicircles (lacking functional p53-specific binding sequence) with a size similar to that of the loop within the hcDNA, indicating that the extreme affinity of p53 for hcDNA is likely due to binding of the protein to the hemicatenane. Only the full-length p53 can form stable ternary complexes with hcDNA and HMGB1.
    PracovištěBiofyzikální ústav
    KontaktJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Rok sběru2005
Number of the records: 1  

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