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Voltammetric behavior of DNA modified with osmium tetroxide 2,2'-bipyridine at mercury electrodes
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SYSNO ASEP 0104640 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Voltammetric behavior of DNA modified with osmium tetroxide 2,2'-bipyridine at mercury electrodes Title Voltametrické chování DNA modifikované komplexem oxidu osmičelého s 2,2'-bipyridinem na rtuťových elektrodách Author(s) Havran, Luděk (BFU-R) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCID
Paleček, Emil (BFU-R) RID, ORCIDSource Title Bioelectrochemistry. - : Elsevier - ISSN 1567-5394
Roč. 63, 1-2 (2004), s. 239-243Number of pages 5 s. Language eng - English Country CH - Switzerland Keywords chemical modification of DNA ; osmium tetroxide complexes ; cyclic voltammetry Subject RIV BO - Biophysics R&D Projects IAA4004108 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KJB4004302 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA204/00/D049 GA ČR - Czech Science Foundation (CSF) GA204/03/0566 GA ČR - Czech Science Foundation (CSF) IBS5004107 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KSK4055109 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5004920 - BFU-R Annotation Osmium tetroxide complexes with nitrogen ligands (L) are probes of DNA structure and electroactive labels of DNA. Here adducts of single-stranded (ss) DNA with osmium tetroxide 2,2'-bipyridine (DNA-Os,bipy) were studied by cyclic voltammetry for the first time. It was found that at neutral pH DNA-Os,bipy produces three redox couples in the potential range between 0 and - 1 V (peaks I-III) and a cathodic peak at about - 1.3 V (peak W). The latter peak decreased with increasing scan rate, and peaks arising from the forward and reverse scans exhibited the same direction, suggesting catalytic nature of the electrode process. We concluded that this peak corresponds to the known differential pulse voltammettic (polarographic) peak of DNA-Os,L adducts for which catalytic hydrogen evolution is responsible. In contrast, currents of cathodic peaks II and III increased almost linearly with increasing scan rate, suggesting involvement of adsorption in the electrode processes. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2005
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