Genetic polymorphism within the Leishmania donovani complex: correlation with geographic origin
1.
SYSNO ASEP
0104486
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Genetic polymorphism within the Leishmania donovani complex: correlation with geographic origin
Title
Genetický polymorfismus uvnitř Leishmania donovani komplexu koreluje s jejich geografickým původem
Author(s)
Zemanová, Eva (PAU-O) Jirků, Milan (PAU-O) Mauricio, I. L. (GB) Miles, M. A. (GB) Lukeš, Julius (PAU-O)
Source Title
American Journal of Tropical Medicine and Hygiene
- ISSN 0002-9637
Roč. 70, č. 6 (2004), s. 613-617
Number of pages
5 s.
Language
eng - English
Country
US - United States
Keywords
genetic polymorphism ; Leishmania donovani ; RAPD
Subject RIV
EB - Genetics ; Molecular Biology
CEZ
AV0Z6022909 - PAU-O, BC-A
Annotation
Random amplified polymorphic DNA (RAPD) was used to detect intraspecific diversity for the Leishmania donovani complex. Fifty-two decameric to 21-meric primers of arbitrary sequence were applied to 15 strains that belong to nine zymodemes. Strains belonging to the species L. major and L. tropica were used as outgroups. A total of 902 amplicons generated by RAPD were scored. Most primers produced species-specific profiles, only 0.6% amplicons were shared by all species, while 4.3% amplicons were common for all 15 strains of the L. donovani complex. Well-supported trees have been constructed, which show a rather strong correlation between the genetic polymorphism of studied strains and their geographic origin. In all obtained trees, L. infantum was paraphyletic. The RAPD profiles suggest that MON-30 belongs to L. donovani. Moreover, the genetic distance between the L. archibaldi strain and other leishmanias does not warrant existence of a separate species
