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Karyotype, sex chromatin and sex chromosome differentiation in the carob moth, Ectomyelois ceratoniae (Lepidoptera : Pyralidae)

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    SYSNO ASEP0104412
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleKaryotype, sex chromatin and sex chromosome differentiation in the carob moth, Ectomyelois ceratoniae (Lepidoptera : Pyralidae)
    TitleKaryotyp, pohlavní chromatin a diferenciace pohlavních chromosomů u zavíječe datlového, Ectomyelois ceratoniae (Lepidoptera : Pyralidae)
    Author(s) Mediouni, J. (TN)
    Fuková, Iva (ENTU-I)
    Frydrychová, Radmila (ENTU-I)
    Dhouibi, M. H. (TN)
    Marec, František (ENTU-I)
    Source TitleCaryologia. - : Firenze University Press - ISSN 0008-7114
    Roč. 57, č. 2 (2004), s. 184-194
    Number of pages10 s.
    Languageeng - English
    CountryIT - Italy
    Keywordscarob moth ; CGH ; genomic hybridization
    Subject RIVEB - Genetics ; Molecular Biology
    R&D ProjectsIAA6007307 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    AnnotationThis paper reports results of the first cytogenetic investigation carried out in the carob moth, Ectomyelois ceratoniae Zeller, the serious polyphagous pest of both stored products and field crops in the Mediterranean basin and Near East regions. The carob moth karyotype consists of 2n=62 chromosomes. In pachytene complements, two NOR bivalents were observed. However, we failed to identify sex chromosomes. In order to differentiate the W-Z sex chromosomes, we employed genomic in situ hybridization (GISH) and comparative genomic hybridization (CGH). GISH detected the W chromosome by strong binding of the Cy3-labelled, female-derived DNA probe. With CGH, both the Cy3-labelled female-derived probe and Fluor-X labelled male-derived probe evenly bound to the W. This suggested that the W is composed predominantly of repetitive DNA sequences occurring scattered in other chromosomes but accumulated in the W chromosome
    WorkplaceInstitute of Entomology (until 2005)
    ContactHelena Musilová, musilova@entu.cas.cz, Tel.: 387 775 216
    Year of Publishing2005
Number of the records: 1  

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