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Monitoring toxicity, DNA damage, and somatic mutations in tobacco plants growing in soil heavily polluted with polychlorinated biphenyls
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SYSNO ASEP 0099652 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Monitoring toxicity, DNA damage, and somatic mutations in tobacco plants growing in soil heavily polluted with polychlorinated biphenyls Title Monitorování toxicity, poškození DNA a somatické mutace u rostlin tabáku rostoucí na půdě s vysokým obsahem polychlorovaných bifenylů Author(s) Gichner, Tomáš (UEB-Q)
Lovecká, P. (CZ)
Kochánková, L. (CZ)
Macková, M. (CZ)
Demnerová, K. (CZ)Source Title Mutation Research - Genetic Toxicology and Environmental Mutagenesis. - : Elsevier - ISSN 1383-5718
Roč. 629, č. 1 (2007), s. 1-6Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords comet assay ; single-cell gel electrophoresis ; gas chromatography Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA521/05/0500 GA ČR - Czech Science Foundation (CSF) Next source Other public resourcesOther public resourcesOther public resources CEZ AV0Z50380511 - UEB-Q (2005-2011) DOI 10.1016/j.mrgentox.2006.11.013 Annotation Heterozygous tobacco (Nicotiana tabacum var. xanthi) plants were cultivated in soil from a dump site highly polluted with polychlorinated biphenyls (PCBs) at Lhenice in South Bohemia, Czech Republic. The total amount of PCBs in the polluted soil, measured by gas chromatography varied from 165 to 265 mg kg(-1) of soil. In tobacco plants cultivated for 8 weeks in the polluted soil the amount of PCBs in the leaves varied from 11 to 28 and in the roots from 104 to 308 mg kg(-1) dry mass. The average leaf area of tobacco plants growing in the PCB-polluted soil was significantly reduced and the DNA damage in leaf nuclei, measured by the comet assay, was slightly but significantly increased compared with controls. The tobacco plants with increased DNA damage showed reduced growth and had distorted leaves. No increase in the frequency of somatic mutations was detected in tobacco plants growing in the PCB-polluted soil. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2008
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