The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

Day, J. G.; Lorenz, M.; Wilding, T.A.; Friedl, T.; Harding, K.; Pröschold, T.; Brennan, D.; Müller, J.; Santos, L. M. A.; Santos, M. F.; Osório, H.C.; Amaral, R.; Lukešová, Alena; Hrouzek, Pavel; Lukeš, Martin; Elster, Josef; Lukavský, Jaromír; Probert, I.; Ryan, M.J.; Benson, E. E.

Number of the records: 1

The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections

1.

SYSNO ASEP	0092230
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections
Title	Využití fyzické a virtuální infrastruktury při validaci metod kryoprezervace řas v mezinárodních sbírkách
Author(s)	Day, J. G. (GB) Lorenz, M. (DE) Wilding, T.A. (GB) Friedl, T. (DE) Harding, K. (GB) Pröschold, T. (GB) Brennan, D. (GB) Müller, J. (DE) Santos, L. M. A. (PT) Santos, M. F. (PT) Osório, H.C. (PT) Amaral, R. (PT) Lukešová, Alena (BC-A)_{RID, ORCID} Hrouzek, Pavel (BC-A) Lukeš, Martin (BC-A) Elster, Josef (BU-J)_{RID, ORCID} Lukavský, Jaromír (BU-J)_{RID, ORCID} Probert, I. (FR) Ryan, M.J. (GB) Benson, E. E. (GB)
Source Title	Cryo-letters. - : CryoLetters LLP - ISSN 0143-2044 Roč. 28, č. 5 (2007), s. 359-376
Number of pages	18 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	cryopreservation ; storage ; culture collections
Subject RIV	EH - Ecology, Behaviour
Next source	Other public resources
CEZ	AV0Z60660521 - UPB-H, BC-A (2005-2011)
	AV0Z60050516 - BU-J (2005-2011)
Annotation	Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P<0.05) between cooling regimes were observed where Mr Frosty was more variable (Inter-Quartile Range being 21.5%, versus 13.0% for CRF samples). Viability assessment using fluorescein diacetate gave significantly (P<0.0001) higher survival than growth in agar with median values being 96% and 89%, respectively.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2008

Number of the records: 1