Pacherník, J. (CZ) Bryja, Vítězslav (UEM-P) Ešner, M. (CZ) Hampl, Aleš (UEM-P) Dvořák, Petr (UEM-P)
Source Title
Physiological Research. - : Fyziologický ústav AV ČR, v. v. i.
- ISSN 0862-8408
Roč. 54, - (2005), s. 257-262
Number of pages
6 s.
Language
eng - English
Country
CZ - Czech Republic
Keywords
Neural differentiation
Subject RIV
FH - Neurology
R&D Projects
GP524/03/P171 GA ČR - Czech Science Foundation (CSF)
LN00A065 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ
AV0Z5039906 - UEM-P
Annotation
Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferating, survival and differentiation activities in a wide range of cell types. Here we show that LIF potentiates retinoic acid-mediated neural induction in pluripotent P19 embryonal carcinoma cells. This activity of LIF was demonstrated by a profounded neural morphology followed by increased expression of neural-specific proteins (N-CAM, III beta-tubulin, and GAP-43), up-regulation of early neural lineage-specific gene Mash-1, and down-regulation of early endoderm-specific genes alfa-fetoprotein and GATA-4. Moreover, LIF also slows growth and increases the level of apoptosis in differentiating cells.
