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Electrochemical microbead-based immunoassay using an (.eta..sup.5./sup.-cyclopentadienyl)tricarbonylmanganese redox marker bound to bovine serum albumin
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SYSNO ASEP 0026099 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Electrochemical microbead-based immunoassay using an (.eta.5-cyclopentadienyl)tricarbonylmanganese redox marker bound to bovine serum albumin Title Použití .eta.5-cyklopentadienyl trikarbonyl manganu jako redox značky bovin sérum albuminu při elektrochemické imunoanalýze s užitím mikronosičů Author(s) Hromadová, Magdaléna (UFCH-W) RID, ORCID, SAI
Salmain, M. (FR)
Fischer-Durand, N. (FR)
Pospíšil, Lubomír (UFCH-W) RID, ORCID
Jaouen, G. (FR)Source Title Langmuir : the ACS journal of surfaces and colloids - ISSN 0743-7463
Roč. 22, - (2005), s. 506-511Number of pages 6 s. Language eng - English Country US - United States Keywords electrochemistry ; bovine serum albumin ; antigen-antibody complex Subject RIV CG - Electrochemistry R&D Projects GA203/03/0821 GA ČR - Czech Science Foundation (CSF) IAA400400505 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC510 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40400503 - UFCH-W (2005-2011) Annotation A first example of the solid-phase immunoassay of a high-wight antigen bovine serum albumin (BSA) using an (.eta.5-cyclopentadienyl)tricarbonylmanganese (cymantrene) redox probe is presented. The electrochemical detection is based on the impedance measurements of a one-electron reversible reduction of the organometallic probe. The microbeadbased immunoassay is discussed for two types of microbeads with different diameters (2.5 and 90 .mu.m) and capabilities to bind the immunoglobulins (2.4 and 10 .mu.g/mg of beads). The use of larger agarose microbeads allows the formation of an antigen-antibody complex at the surface of microbeads directly dispersed in the analyzed solution. No additional separation step is necessary for the electrochemical competitive immunoassay analysis of BSA. The presence of agarose beads in the analyzed solution has no effect on the electrochemical signal from labeled BSA released from the antigen-antibody complex. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2006
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