NANODIAMONDS ENCAPSULATED IN NANOFIBERS AS A NEW STRATEGY FOR MICRORNA-BASED THERAPY OF PROSTATE CANCER

0522949 - ÚEM 2020 RIV CZ eng C - Conference Paper (international conference)
Bitti, Giuseppe - Divín, R. - Benson, Veronika - Filová, Eva - Amler, E.
NANODIAMONDS ENCAPSULATED IN NANOFIBERS AS A NEW STRATEGY FOR MICRORNA-BASED THERAPY OF PROSTATE CANCER.
NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application. Ostrava: Tanger Ltd., 2019, s. 348-353. ISBN 978-808729489-5.
[NANOCON 2018 -International Conference on Nanomaterials - Research and Application /10./. Brno (CZ), 17.10.2018-19.10.2018]
R&D Projects: GA MŠMT(CZ) LO1309; GA MŠMT(CZ) LO1508
Institutional support: RVO:68378041 ; RVO:61388971
Keywords : nanodiamonds * nanofibers * gene therapy
OECD category: Biomaterials (as related to medical implants, devices, sensors); Biochemistry and molecular biology (MBU-M)

Given the miRNA-34 family role as tumor suppressor in cancer, it was showed that using miRNA-34a as a new anticancer agent has a great therapeutic potential. Unfortunately, biopharmaceutical issues, rapid degradation in biological fluids, poor uptake into cells and not specific distribution into the body, prevent the use of wildtype non-coding RNA by systemic administration. To overcome these limits, in our previous study we developed a new therapeutic concept based on nanotechnology delivery of miRNA-34a into prostate cancer using fluorescent nanodiamond particles coated with polyethylenimine. In the current study, in order to achieve a more conservative, stable and controlled release of miRNA-34a, we designed a system based on nanodimaonds- miRNA34a complexes encapsulated into nanofibers. We successfully encapsulate nanodiamonds into fibers of polycaprolactione, a biodegradable polymer with a slow degradation rate and a high cellular adhesion, using an electropsinning process. We cultivated prostate cancer cells (PC3 and DU145) on nanofibers scaffolds and we analyzed the presence of nanodiamonds in cells using confocal microscopy. Moreover, in these prostate cancer cell lines we observed a replacement of miRNA-34a via real-time PCR and a decreased viability testing the metabolic activity. Our preliminar results suggest a higher increased and stable mid-term replacement of oncosuppressor miRNA-34a in prostate cancer when the nanodiamonds-miRNA complexes are delivered encapsulating them in nanofibers. Therefore we provided an effective strategy for cancer therapy ready to be tested for in vivo experiments.
Permanent Link: http://hdl.handle.net/11104/0307355