Diamond aminated with plasma polymer as biocompatible substrate for protein attachment

0521839 - FZÚ 2020 RIV UA eng A - Abstract
Artemenko, Anna - Kozak, Halyna - Ukraintsev, Egor - Kromka, Alexander
Diamond aminated with plasma polymer as biocompatible substrate for protein attachment.
Proceedings of abstracts of the International Conference Nanobiophysics: fundamental and applied aspects /6./. Kiev: Press Service of NAS of Ukraine, 2019. s. 10-10. ISBN 978-966-02-9007-5.
[International Conference Nanobiophysics: fundamental and applied aspects /6./. 01.10.2019-04.10.2019, Kiev]
R&D Projects: GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : nanocrystalline diamond
OECD category: Biophysics

Nanocrystalline diamond (NCD) films are known as a highly attractive material for biosensing applications due to their unique semiconducting properties. In this study, we investigate the immobilization of bovine serum albumin (BSA) molecules on the surface of flat and nanoporous diamond films terminated by hydrogen, oxygen or amine-containing plasma polymer (thickness 7 nm). GAR-FTIR and XPS measurements confirmed that the concentration of amine-containing bonds after the BSA attachment depends on the NCD surface termination. We found that the amine-containing polymer provides a strong interlink between BSA protein and the NCD film while preserving its globular conformation and probably also its biochemical functionality. This work was supported by Operational Programme Research, Development and Education financed by European Structural and Investment Funds and the Czech Ministry of Education, Youth and Sports (Project No. SOLID21 - CZ.02.1.01/0.0/0.0/16_019/0000760).

Permanent Link: http://hdl.handle.net/11104/0306398