Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough

0518352 - MBÚ 2020 RIV GB eng J - Journal Article
Beznosková, Petra - Pavlíková, Zuzana - Zeman, Jakub - Aitken, C.E. - Valášek, Leoš Shivaya
Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough.
Nucleic Acids Research. Roč. 47, č. 12 (2019), s. 6339-6350. ISSN 0305-1048. E-ISSN 1362-4962
R&D Projects: GA ČR(CZ) GA18-02014S
Institutional support: RVO:61388971
Keywords : Frameshifting * Codon * Ribosomal frameshifting
OECD category: Microbiology
Impact factor: 11.502, year: 2019
Method of publishing: Open access
https://academic.oup.com/nar/article/47/12/6339/5487265

Stop codon readthrough-the decoding of a stop codon by a near-cognate tRNA-is employed by viruses to balance levels of enzymatic and structural proteins and by eukaryotic cells to enable isoform-specific protein synthesis in response to external stimuli. Owing to the prevalence of premature termination codons in human disease, readthrough has emerged as an attractive therapeutic target. A growing list of various features, for example the +4 nucleotide immediately following the stop codon, modulate readthrough levels, underscoring the need for systematic investigation of readthrough. Here, we identified and described a complete group of yeast tRNAs that induce readthrough in the stop-codon tetranucleotide manner when overexpressed, designated readthrough-inducing tRNAs (rti-tRNAs). These rti-tRNAs are the keystones of YARIS (yeast applied readthrough inducing system), a reporter-based assay enabling simultaneous detection of readthrough levels at all twelve stop-codon tetranucleotides and as a function of the complete set of rti-tRNAs. We demonstrate the utility of YARIS for systematic study of translation readthrough by employing it to interrogate the effects of natural rti-tRNA modifications, as well as various readthrough-inducing drugs (RTIDs). This analysis identified a variety of genetic interactions demonstrating the power of YARIS to characterize existing and identify novel RTIDs. © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
Permanent Link: http://hdl.handle.net/11104/0303510