The serine protease inhibitors and SERPINE1/2 disrupt prostaglandin E2 production and hyaluronic acid retention in FSH-stimulated pig cumulus-oocyte complexes

0517323 - ÚŽFG 2020 RIV US eng J - Journal Article
Blaha, Milan - Nevoral, J. - Procházka, Radek
The serine protease inhibitors and SERPINE1/2 disrupt prostaglandin E2 production and hyaluronic acid retention in FSH-stimulated pig cumulus-oocyte complexes.
Reproduction in Domestical Animals. Roč. 54, Supplement 4 (2019), s. 65-68. ISSN 0936-6768. E-ISSN 1439-0531
R&D Projects: GA MZe(CZ) QJ1510138; GA MŠMT EF15_003/0000460
Institutional support: RVO:67985904
Keywords : cumulus cells * follicle stimulating hormone * plasmin
OECD category: Developmental biology
Impact factor: 1.641, year: 2019
Method of publishing: Open access
https://onlinelibrary.wiley.com/doi/full/10.1111/rda.13497

Contents The serine proteases, tissue- and urokinase-type plasminogen activators (PLAT and PLAU) and their inhibitors SERPINE1/2 are regulators of plasminogen to plasmin conversion. They are widely expressed in ovarian tissues, including granulosa and cumulus cells, and their expression is regulated by gonadotropins. The aim of this work was to assess the effect of serine protease inhibitors (aprotinin and AEBSF) and SERPINE1/2 on FSH-induced cumulus cell expansion, the production of prostaglandin E2 (PGE2) and retention of hyaluronic acid (HA) in expanding cumulus. The serine protease activity proved to be essential for the production of PGE2 and also for the retention of HA, the inhibition of plasminogen activators by SERPINE1/2 had the same effect. Collectively, these data indicate that plasmin is required for proper function of expanding cumulus cells in vitro and presumably also in vivo in the pre-ovulatory follicles.
Permanent Link: http://hdl.handle.net/11104/0302648