Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly

0511982 - ÚŽFG 2020 RIV CH eng J - Journal Article
Iannucci, A. - Altmanová, Marie - Ciofi, C. - Ferguson-Smith, M. A. - Pereira, J. - Řehák, I. - Stanyon, R. - Velenský, P. - Rovatsos, Michail - Kratochvíl, L. - Johnson Pokorná, Martina
Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly.
Cytogenetic and Genome Research. Roč. 157, 1-2 (2019), s. 123-131. ISSN 1424-8581. E-ISSN 1424-859X
Institutional support: RVO:67985904
Keywords : chromosome flow sorting * laser capture * microdissection
OECD category: Zoology
Impact factor: 1.114, year: 2019
Method of publishing: Limited access
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=41048410983

We developed new tools to build a high-quality chromosomal map of the Komodo dragon (Varanus komodoensis) available for cross-species phylogenomic analyses. First, we isolated chromosomes by flow sorting and determined the chromosome content of each flow karyotype peak by FISH. We then isolated additional Komodo dragon chromosomes by microdissection and amplified chromosome-specific DNA pools. The chromosome-specific DNA pools can be sequenced, assembled, and mapped by next-generation sequencing technology. The chromosome-specific paint probes can be used to investigate karyotype evolution through cross-species chromosome painting. Overall, the set of chromosome-specific DNA pools of V. komodoensis provides new tools for detailed phylogenomic analyses of Varanidae and squamates in general.
Permanent Link: http://hdl.handle.net/11104/0302212