beta-N-Acetylhexosaminidases-the wizards of glycosylation

0509795 - MBÚ 2020 RIV DE eng J - Journal Article
Bojarová, Pavla - Bruthans, J. - Křen, Vladimír
beta-N-Acetylhexosaminidases-the wizards of glycosylation.
Applied Microbiology and Biotechnology. Roč. 103, č. 19 (2019), s. 7869-7881. ISSN 0175-7598. E-ISSN 1432-0614
R&D Projects: GA MŠMT(CZ) LTC17005; GA MŠMT(CZ) LTC18041
Institutional support: RVO:61388971
Keywords : beta-N-acetylhexosaminidase * Carbohydrate * Enzymatic synthesis
OECD category: Biochemistry and molecular biology
Impact factor: 3.530, year: 2019
Method of publishing: Limited access
https://link.springer.com/article/10.1007%2Fs00253-019-10065-0

beta-N-Acetylhexosaminidases (EC 3.2.1.52) are a unique family of glycoside hydrolases with dual substrate specificity and a particular reaction mechanism. Though hydrolytic enzymes per se, their good stability, easy recombinant production, absolute stereoselectivity, and a broad substrate specificity predestine these enzymes for challenging applications in carbohydrate synthesis. This mini-review aims to demonstrate the catalytic potential of beta-N-acetylhexosaminidases in a range of unusual reactions, processing of unnatural substrates, formation of unexpected products, and demanding reaction designs. The use of unconventional media can considerably alter the progress of transglycosylation reactions. By means of site-directed mutagenesis, novel catalytic machineries can be constructed. Glycosylation of difficult substrates such as sugar nucleotides was accomplished, and the range of afforded glycosidic bonds comprises unique non-reducing sugars. Specific functional groups may be tolerated in the substrate molecule, which makes beta-N-acetylhexosaminidases invaluable allies in difficult synthetic problems.
Permanent Link: http://hdl.handle.net/11104/0300424