EFFECT OF BONE MORPHOGENETIC PROTEIN-15 ON GONADOTROPIN-STIMULATED SYNTHESIS OF HYALURONAN AND PROGESTERONE IN PORCINE OVARIAN FOLLICLE

0506398 - ÚŽFG 2020 RIV PL eng J - Journal Article
Nagyová, Eva - Němcová, Lucie - Bujnaková Mlynarčíková, A. - Blaha, Milan - Procházka, Radek - Scsuková, S.
EFFECT OF BONE MORPHOGENETIC PROTEIN-15 ON GONADOTROPIN-STIMULATED SYNTHESIS OF HYALURONAN AND PROGESTERONE IN PORCINE OVARIAN FOLLICLE.
Journal of Physiology and Pharmacology. Roč. 68, č. 5 (2017), s. 683-691. ISSN 0867-5910
R&D Projects: GA MŠMT EF15_003/0000460
Institutional support: RVO:67985904
Keywords : bone morphogenetic protein-15 * cumulus extracellular matrix * granulosa cells
OECD category: Developmental biology
Impact factor: 2.478, year: 2017
Method of publishing: Limited access
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=11137804280

Bone morphogenetic protein-15 (BMP-15), an oocyte-derived growth factor, has been shown to play integral roles in regulation of ovarian follicular function in mammals. Despite the recognition of the physiological importance of the BMP system in regulation of gonadotropin action in the ovary, molecular mechanisms of BMP-15 effect on oocyte and somatic follicular cell functions remain poorly understood. The objective of this study was to determine the effect of BMP-15 on the FSH/LH-stimulated synthesis of hyaluronan (HA) by oocyte annulus complexes (OCC) and progesterone by OCC and granulosa cells (GC) in the presence or absence of serum using primary porcine cultures. In addition, the effect of BMP-15 on oocyte maturation- and steroidogenesis-related transcripts after 4, 8, 16, and 24 hours of cultivation was evaluated using real-time RT-PCR. We demonstrated that the FSH/LH-induced cumulus expansion was accompanied by a significant increase in CD44, PTGS2, CYP11A1 (at 4 h) and AREG, HAS2, TIVFAIP6, STAR (at 8 h) mRNAs. While FSH/LH-stimulated total HA synthesis by OCC was not affected by BMP-15 in serum-supplemented medium, its retention within the complex was significantly increased after the action of BMP-15 in comparison to FSH/LH alone (P < 0.001, 65% versus 35%. respectively). Moreover, we detected a significant increase in the expression of AREG and TNFAIP6 (both at 16 h), and CYPIIA1 (at 24 h) in FSH/LH-stimulated OCC due to the action of BMP-15 compared to complexes cultured only with FSH/LH. In the presence of serum, BMP-15 markedly increased FSH/LH-stimulated progesterone secretion by OCC (about 69%) and induced a significant decrease in FSH/LH-induced progesterone release by GC (about 35%) compared to FSH/LH alone. The present results indicate that the addition of BMP-15 to the gonadotropin-stimulated OCC cultured in serum-supplemented medium might improve oocyte-cumulus maturation.
Permanent Link: http://hdl.handle.net/11104/0297641