Enucleolation and nucleolus transfer in mammalian oocytes and zygotes

0504368 - ÚŽFG 2020 RIV ES eng J - Journal Article
Benc, M. - Fulka Jr., J. - Strejček, F. - Morovič, M. - Murín, Matěj - Martínková, S. - Jettmarová, D. - Laurinčík, Jozef
Enucleolation and nucleolus transfer in mammalian oocytes and zygotes.
International Journal of Developmental Biology. Roč. 63, 3-5 (2019), s. 253-258. ISSN 0214-6282. E-ISSN 1696-3547
R&D Projects: GA MŠMT EF15_003/0000460
Institutional support: RVO:67985904
Keywords : nucleous * micromanipulation * oocyte
OECD category: Developmental biology
Impact factor: 1.105, year: 2019
Method of publishing: Open access
http://www.ijdb.ehu.es/web/paper/190002mb/enucleolation-and-nucleolus-transfer-in-mammalian-oocytes-and-zygotes

The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.
Permanent Link: http://hdl.handle.net/11104/0296017