Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery

0499815 - MBÚ 2019 RIV US eng J - Journal Article
Benson, Veronika - Neuhoferová, Eva
Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery.
Drug Designing & Intellectual Properties International Journal. Roč. 1, č. 4 (2018), s. 112-117. ISSN 2637-4706
R&D Projects: GA MZd(CZ) NV15-33094A
Institutional support: RVO:61388971
Keywords : Micro-RNA * RNA interference * Nano Diamond
OECD category: Microbiology

Micro RNAs are short non-coding RNA molecules, which are important regulators of cell functions. Deregulation of certain micro RNA level is contributing factor to tumor formation and progression. Possible gene therapy by modulation of the micro RNA level is currently considered. Recently it has been proved that nano diamonds coated with PEI serve as efficient transfection and monitoring system. The aim of our work was to deliver micro RNA inhibitor and micro RNA mimic sequences into specialized mammalian
cells using fluorescent nano diamond particles and to test the nano complex uptake and cell compatibility during primary in vivo application. Short RNA molecules were bound to the PEI coated nano diamonds, transported into the colon cancer cell line in vitro and observed in a confocal microscope. The miRNA mimic coated nano diamonds were used for complex uptake and toxicity study in peritoneal macrophages and splenocytes after ex vivo stimulation or in vivo administration. Due to the possibility of continuous
monitoring of carrier internalization, its transfection efficacy, and cell compatibility, the fluorescent nano diamonds represent a suitable RNA carrier with a potential use in gene therapy.
Permanent Link: http://hdl.handle.net/11104/0292005