HPLC-ED/UV for determination of vanillylmandelic acid in human urine after solid phase extraction

0498465 - ÚFCH JH 2019 RIV CZ eng C - Conference Paper (international conference)
Němečková-Makrlíková, Anna - Dejmková, H. - Navrátil, Tomáš - Barek, J. - Vyskočil, V.
HPLC-ED/UV for determination of vanillylmandelic acid in human urine after solid phase extraction.
Proceedings of the 14th International Students Conference “Modern Analytical Chemistry”. Praha: Univerzita Karlova v Praze, Přírodovědecká fakulta, 2018 - (Nesměrák, K.), s. 82-86. ISBN 978-80-7444-059-5.
[International Students Conference “Modern Analytical Chemistry” /14./. Praha (CZ), 20.09.2018-21.09.2018]
R&D Projects: GA ČR GA17-03868S
Institutional support: RVO:61388955
Keywords : HPLC * solid phase extraction * tumor biomarker
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

HPLC with electrochemical and spectrophotometric detection (ED/UV) after solid phase extraction (SPE) was used for determination of vanillylmandelic acid in human urine. HPLC-ED was performed at a glassy carbon electrode in a “wall-jet” arrangement in acetate-phosphate buffer at pH = 2.5 and gradient elution (increasing content of
acetonitrile from 5 to 25% in 10 minutes) was used. Optimized parameters were following: flow rate of mobile phase 1 mL min−1, detection potential +1.1 V, detection wavelength 279 nm, injected volume 20 μL. Dependence of the peak current on the analyte concentration was linear in the concentration range from 10 to 150 μmol L−1, with obtained limits of detection 2.6 μmol L−1 (calculated from peak height) and 1.9 μmol L−1 (calculated from peak area) for HPLC-ED, and 11.0 μmol L−1 (calculated from peak height) and 9.8 μmol L−1 (calculated from peak area) for HPLC-UV.
Permanent Link: http://hdl.handle.net/11104/0290828