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Localization of RNA and translation in the mammalian oocyte and embryo

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    0489565 - ÚŽFG 2019 RIV US eng J - Journal Article
    Jansová, Denisa - Tětková, Anna - Končická, Markéta - Kubelka, Michal - Šušor, Andrej
    Localization of RNA and translation in the mammalian oocyte and embryo.
    PLoS ONE. Roč. 13, č. 3 (2018), č. článku e0192544. ISSN 1932-6203. E-ISSN 1932-6203
    R&D Projects: GA ČR GA13-12291S; GA ČR GA15-22765S; GA MŠMT EF15_003/0000460
    Institutional support: RVO:67985904
    Keywords : RNA localization * mammalian oocyte
    OECD category: Biochemistry and molecular biology
    Impact factor: 2.776, year: 2018

    The tight correlation between mRNA distribution and subsequent protein localization and function indicate a major role for mRNA localization within the cell. RNA localization, followed by local translation, presents a mechanism for spatial and temporal gene expression regulation utilized by various cell types. However, little is known about mRNA localization and translation in the mammalian oocyte and early embryo. Importantly, fully-grown oocyte becomes transcriptionally inactive and only utilizes transcripts previously synthesized and stored during earlier development. We discovered an abundant RNA population in the oocyte and early embryo nucleus together with RNA binding proteins. We also characterized specific ribosomal proteins, which contribute to translation in the oocyte and embryo. By applying selected markers to mouse and human oocytes, we found that there might be a similar mechanism of RNA metabolism in both species. In conclusion, we visualized the localization of RNAs and translation machinery in the oocyte, that could shed light on this terra incognita of these unique cell types in mouse and human.
    Permanent Link: http://hdl.handle.net/11104/0283964

     
     
Number of the records: 1  

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