Voltammetric Determination of Tumor Biomarkers using Flow Injection Analysis with Amperometric Detection

0479846 - ÚFCH JH 2018 RIV CZ eng C - Conference Paper (international conference)
Makrlíková, Anna - Matysik, F.-M. - Barek, J. - Vyskočil, V.
Voltammetric Determination of Tumor Biomarkers using Flow Injection Analysis with Amperometric Detection.
Proceedings of the 13th International Students Conference ”Modern Analytical Chemistry”. Prague: Charles University in Prague, Faculty of Science, 2017 - (Nesměrák, K.), s. 7-11. ISBN 978-80-7444-052-6.
[International Students Conference ”Modern Analytical Chemistry” /13./. Prague (CZ), 21.09.2017-22.09.2017]
Institutional support: RVO:61388955
Keywords : flow injection analysis * 5-hydroxyindole-3-acetic acid * homovanillic acid
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Three tumor biomarkers (homovanillic acid, vanillylmandelic acid,
and 5-hydroxyindole-3-acetic acid) have been determined by flow
injection analysis with amperometric detection at screen-printed
carbon electrodes in optimum medium of Britton-Robinson buffer
(0.04 mol l-1, pH = 2.0). Dependences of the peaks current on the
concentration of biomarkers were linear in the tested concentration
region from 0.05 to 100 mu mol l-1, with the limits of detection of
0.065 mu mol l-1 for homovanillic acid, 0.053 mu mol l-1 for vanillylmandelic
acid, and 0.033 mu mol l-1 for 5-hydroxyindole-3-acetic acid (calculated
from heights), and 0.024 mu mol 1-1 for homovanillic acid,
0.020 mu mol l-1 forvanillylmandelic acid, and 0.012 mu mol l-1 for 5-hydroxyindole-3-acetic acid (calculated from areas), respectively.
Permanent Link: http://hdl.handle.net/11104/0275772