Determination of 5-hydroxyindole-3-acetic acid using Flow Injection Analysis with Electrochemical Detection

0475141 - ÚFCH JH 2018 RIV CZ eng C - Conference Paper (international conference)
Makrlíková, Anna - Matysik, F.-M. - Navrátil, Tomáš - Barek, J. - Vyskočil, V.
Determination of 5-hydroxyindole-3-acetic acid using Flow Injection Analysis with Electrochemical Detection.
XXXVII. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference. Ústí nad Labem: Best servis, 2017 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), s. 137-141. ISBN 978-80-905221-5-2.
[Moderní elektrochemické metody /37./. Jetřichovice (CZ), 15.05.2017-19.05.2017]
R&D Projects: GA ČR(CZ) GA17-03868S
Institutional support: RVO:61388955
Keywords : Amperometric detection * Flow injection analysis * 5-Hydroxyindole-3-acetic acid
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Tumour biomarker, 5-hydroxyindole-3-acetic acid, has been determined at screen-printed carbon electrodes using flow injection analysis with amperometric detection. Britton-Robinson buffer (0.04 mol-l-1 , pH 2.0) was used as an optimum carrier solution.
Dependence of the peak current on the analyte concentration was linear in the whole tested
concentration range (from 0.5 to 100 mikromol-l-1) with limits of detection 0.033 mikromol-l-1
(calculated from peak heighst) and 0.012 mikromol-l-1 (calculated from peak areas).
Permanent Link: http://hdl.handle.net/11104/0271996