Flow Analysis and Sorting of Plant Chromosomes

0471598 - ÚEB 2017 RIV US eng J - Journal Article
Vrána, Jan - Cápal, Petr - Šimková, Hana - Karafiátová, Miroslava - Čížková, Jana - Doležel, Jaroslav
Flow Analysis and Sorting of Plant Chromosomes.
Current protocols in cytometry. Roč. 78, Oct 10 (2016), 5.3.1-5.3.43. ISSN 1934-9300
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : cell cycle synchronization * chromosome genomics * chromosome isolation
Subject RIV: EB - Genetics ; Molecular Biology

Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner, starting with the induction of cell cycle synchrony and accumulation of dividing cells in mitotic metaphase, and continues with the preparation of suspensions of intact mitotic chromosomes, flow analysis and sorting of chromosomes, and finally processing of the sorted chromosomes. Each step of the protocol is described in detail as some procedures have not been used widely. Supporting histograms are presented as well as hints on dealing with plant material; the utility of sorted chromosomes for plant genomics is also discussed.
Permanent Link: http://hdl.handle.net/11104/0268958