Selected Ga-68-siderophores versus Ga-68-colloid and Ga-68-citrate: biodistribution and small animal imaging in mice

0446378 - ÚEB 2016 RIV CZ eng J - Journal Article
Petřík, M. - Vlčková, A. - Nový, Z. - Urbánek, Lubor - Haas, H. - Decristoforo, C.
Selected Ga-68-siderophores versus Ga-68-colloid and Ga-68-citrate: biodistribution and small animal imaging in mice.
Biomedical Papers. Roč. 159, č. 1 (2015), s. 60-66. ISSN 1213-8118. E-ISSN 1804-7521
R&D Projects: GA MŠMT(CZ) LO1304
Institutional support: RVO:61389030
Keywords : gallium-68 * siderophores * colloid
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.924, year: 2015

Background. Ga-68-triacetylfusarinine C (TAFC) and Ga-68-ferrioxamine E (FOXE) show great potential to be used as highly sensitive and selective tracers for Aspergillus infection imaging. Here we report on a comparison of the ex vivo biodistribution and small animal imaging of Ga-68-TAFC and Ga-68-FOXE versus Ga-68-colloid and Ga-68-citrate as unspecific control in mice. Methods. The radiochemical purity of tested Ga-68 labelled tracers was determined by RP-HPLC or ITLC-SG. Ex vivo biodistribution was studied in normal DBA/2 mice 30 min and 90 min p.i_ Static and dynamic imaging were performed using mu PET/CT. Results. Ga-68-TAFC and Ga-68-FOXE showed rapid renal excretion and low blood values even 90 min p.i_ Ga-68-TAFC showed almost no retention in other organs while 68Ga-FOXE displayed some uptake in gastrointestinal tract. Ga-68-colloid and Ga-68-citrate revealed significantly different ex vivo biodistribution. Ga-68-colloid showed pronounced radioactivity retention in the liver, while Ga-68-citrate displayed high blood values and significant retention of radioactivity in highly perfused organs. Conclusions. From the results, both Ga-68-TAFC and Ga-68-FOXE have excellent and significantly different in vivo behaviour compared to Ga-68-colloid and Ga-68-citrate. Ga-68-TAFC in particular confirmed its great potential use as a specific tracer for Aspergillus infection imaging.
Permanent Link: http://hdl.handle.net/11104/0248384